Bile salt secretion by hepatocytes incubated with bile salts and liposomes or low density lipoproteins
Identifieur interne : 000146 ( France/Analysis ); précédent : 000145; suivant : 000147Bile salt secretion by hepatocytes incubated with bile salts and liposomes or low density lipoproteins
Auteurs : Thierry Clerc [France] ; Véronique Sbarra [France] ; Danielle Botta-Fridlund [France] ; Huguette Lafont [France] ; Paul Pak-Leung [France] ; André Gauthier [France] ; Françoise Chanussot [France]Source :
- Life Sciences [ 0024-3205 ] ; 1994.
Abstract
The purpose of this work was to determine the effect of exogenous unesterified cholesterol provided in either artificial liposomes or LDL on bile salt synthesis by isolated rat hepatocytes. Rates of de novo synthesis were determined in the presence of 300 or 600 μM taurocholate, 600 μM taurodehydrocholate, cholate, deoxycholate or chenodeoxycholate. There was no significant difference between the cholesterol uptake by hepatocytes when the degree of hydrophobicity of the bile salts changed (cholate vs deoxycholate or chenodeoxycholate). Compared to taurocholate, taurodehydrocholate lowered the hepatic incorporation of unesterified cholesterol for the first 60 minutes; compared to control, taurocholate stimulated the cholesterol incorporation for the first 20 minutes. A possible explanation for this finding would be an interaction between bile salts and exogenous cholesterol, depending on the kind of conjugated bile salt. Taurocholate increased the exchange of cholesterol between liposomes or LDL and hepatocyte membranes. It resulted in a significant increase of bile salt synthesis and secretion. This phenomenon was not observed with taurodehydrocholate.
Url:
DOI: 10.1016/0024-3205(94)00922-8
Affiliations:
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ISTEX:6C66E86C059AE59B58614075FF71E52F33316114Le document en format XML
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Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Pak Leung, Paul" sort="Pak Leung, Paul" uniqKey="Pak Leung P" first="Paul" last="Pak-Leung">Paul Pak-Leung</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Service d'Hépatologie, Hôpital de la Conception, 147 boulevard Baille, 13005 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Gauthier, Andre" sort="Gauthier, Andre" uniqKey="Gauthier A" first="André" last="Gauthier">André Gauthier</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Service d'Hépatologie, Hôpital de la Conception, 147 boulevard Baille, 13005 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when="1994">1994</date><biblScope unit="volume">56</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="277">277</biblScope><biblScope unit="page" to="286">286</biblScope></imprint><idno type="ISSN">0024-3205</idno></series><idno type="istex">6C66E86C059AE59B58614075FF71E52F33316114</idno><idno type="DOI">10.1016/0024-3205(94)00922-8</idno><idno type="PII">0024-3205(94)00922-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0024-3205</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The purpose of this work was to determine the effect of exogenous unesterified cholesterol provided in either artificial liposomes or LDL on bile salt synthesis by isolated rat hepatocytes. Rates of de novo synthesis were determined in the presence of 300 or 600 μM taurocholate, 600 μM taurodehydrocholate, cholate, deoxycholate or chenodeoxycholate. There was no significant difference between the cholesterol uptake by hepatocytes when the degree of hydrophobicity of the bile salts changed (cholate vs deoxycholate or chenodeoxycholate). Compared to taurocholate, taurodehydrocholate lowered the hepatic incorporation of unesterified cholesterol for the first 60 minutes; compared to control, taurocholate stimulated the cholesterol incorporation for the first 20 minutes. A possible explanation for this finding would be an interaction between bile salts and exogenous cholesterol, depending on the kind of conjugated bile salt. Taurocholate increased the exchange of cholesterol between liposomes or LDL and hepatocyte membranes. It resulted in a significant increase of bile salt synthesis and secretion. This phenomenon was not observed with taurodehydrocholate.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Clerc, Thierry" sort="Clerc, Thierry" uniqKey="Clerc T" first="Thierry" last="Clerc">Thierry Clerc</name></region><name sortKey="Botta Fridlund, Danielle" sort="Botta Fridlund, Danielle" uniqKey="Botta Fridlund D" first="Danielle" last="Botta-Fridlund">Danielle Botta-Fridlund</name><name sortKey="Chanussot, Francoise" sort="Chanussot, Francoise" uniqKey="Chanussot F" first="Françoise" last="Chanussot">Françoise Chanussot</name><name sortKey="Gauthier, Andre" sort="Gauthier, Andre" uniqKey="Gauthier A" first="André" last="Gauthier">André Gauthier</name><name sortKey="Lafont, Huguette" sort="Lafont, Huguette" uniqKey="Lafont H" first="Huguette" last="Lafont">Huguette Lafont</name><name sortKey="Pak Leung, Paul" sort="Pak Leung, Paul" uniqKey="Pak Leung P" first="Paul" last="Pak-Leung">Paul Pak-Leung</name><name sortKey="Sbarra, Veronique" sort="Sbarra, Veronique" uniqKey="Sbarra V" first="Véronique" last="Sbarra">Véronique Sbarra</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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